Microbiological Enumeration of Pathogens Associated with Urinary Tract Infection: Current Scenario and Its Medical Relevance
Pooja Singh *
Department of Zoology, DBS College Dehradun, Uttrakhand, India. and DNA Labs A Centre for Applied Sciences, Dehradun, Uttrakhand, India.
Jyoti Chandola
DNA Labs A Centre for Applied Sciences, Dehradun, Uttrakhand, India. and Department of Botany, DBS College Dehradun, Uttrakhand, India.
Manoj Singh Shahi
DNA Labs A Centre for Applied Sciences, Dehradun, Uttrakhand, India. and Department of Microbiology, Sai Institute of Paramedical and Allied Sciences, Dehradun, Utrakhand, India.
Narotam Sharma
DNA Labs A Centre for Applied Sciences, Dehradun, Uttrakhand, India.
Komal Gupta
DNA Labs A Centre for Applied Sciences, Dehradun, Uttrakhand, India.
Sandeep Singh
DNA Labs A Centre for Applied Sciences, Dehradun, Uttrakhand, India.
*Author to whom correspondence should be addressed.
Abstract
Background: Urinary tract infection (UTI) is the most common among community acquired infections worldwide affecting men as well as women, many reports suggested that uropathogens are becoming more resistant towards antibiotics, which can cause various problems during treatment.
Methods: In this study, 48 samples were collected from the suspected patients and these samples were subjected to various clinical evaluations.
Results: Among them 29 were females and 19 were males, in the studied cases E. coli (48.3%) was most frequently observed pathogens, S. aureus (29.03%).Among antibiotics, Tigecyclin (TGC) was the most effective antibiotic against all the three isolated bacteria, about 100% of bacteria showed the sensitivity towards it. E. coli was found to be the most resistant bacteria against Tetracycline (TET) (66.6%) followed by Amoxiclav (AMC) (60%), Fosfomycin (Fo) (53.3%), Piperacilllin (PIP) (53.3%), Cefepime (CPM) (53.3%). Among K. pneumonia isolates had shown resistance against Tetracycline (TET) (57.1%), Fosfomycin (FO) (57.1%), Piperacillin (PI) (57.1%), Cephalexin (CZ) (57.1%) and Amikacin (AK) (57.1%). Staphylococcus aureus had shown maximum resistance against Tetracycline (TET) (66.5%), Co-amoxiclav (AMC) (66.5%) followed by Chloramphenicol (55.4%) and Cefepime (CPM) (55.4%).
Conclusion: In conclusion UTI infection rates are higher among females, E. coli was the most causative agents of UTI and the emergence of resistance in bacteria is probably due to the overuse of antibiotics. Antibiotic Tigecyclin could be the drug of choice for the treatment of UTI.
Keywords: Urinary tract infection, uropathogens, clinical evaluation, antibiotics, emergence.