The Study of Sectional Anatomy of Sheep and Human Brain by Various Staining Methods
Asian Journal of Medicine and Health,
Five different staining methods were done on the sheep brain compared and the best method was employed to stain the human brain, Alstons staining method is found to be the best among the five staining method, which shows excellent differentiation of grey and white matter. Alston’s method has low shrinkage percentage and the time taken for this procedure is around 8 hrs 30 min. When compared with the unstained section with the stained section show pronounced grey matter. Learning neuroanatomy with wet brain section is difficult in unstained brain specimens, with the induction of staining method in the nueroanatomical structures will be helpful in differentiating the grey and white matter of brain. With the coronal and axial sections in this study various macroscopic neuroanatomical structures can be studied easily.
- alstons stain
- mulligan stain
How to Cite
Alfred Riepertinger, Fixation of the human brain for plastination: special considerations. J Int Soc Plastination. 1988;2(1):8-12.
Chytas D, Piagkou M, Johnson EO, Tsakotos G, Mazarakis A, Babis GC, Nikolaou VS, Kaseta MK, Natsis K. Outcomes of the use of plastination in anatomy education: current evidence. Surg Radiol Anat. 2019;41(10):1181-1186.
Sora, Constantin & Latorre, Rafael & Baptista, Carlos & Lopez Albors, Octavio. Plastination—A scientific method for teaching and research. Anatomia, Histologia, Embryologia. 2019:48.
Latorre R, Bainbridge D, Tavernor A, López Albors O. Plastination in Anatomy Learning: An Experience at Cambridge University. J Vet Med Educ. 2016 ;43(3):226-34.
Neha hassan patankar and Vinod m. Choudhari., Plastination an incipient way of learning anatomy-a review., World Journal of Pharmaceutical and Medical Research. 2019;7(8):164 – 168.
Henry RW, History & principles of dehydration.J Int Soc Plastination. 2001; 16:31;41:45-50.
Henry RW, Brown MA, Henry CL, Reed RB, Dehydration: 6000 BC., 19th century, First quarter century of plastination and the New Millennium. J Int Soc Plastination. 2001;16: 31-41:45-50.
Mulligan JH .A method study of staining the brain for macroscopic study. J Anat London. 1931;65:468 -472.
Lalita suriyaprapadilok, Boonsirm Withyachumnarnkul. Plastination of stained sections of the human brain: Comparison between different staining methods. J Int Soc Plastination. 1997;12(1):27- 32.
Norbert Ulfig. Staining of human Fetal and adult brain slices combined with subsequent plastination. J Int Soc Plastination. 1990;4:33- 38.
Baeres FMM. Plastination of Dissected Brain Specimens and Mulligan-stained sections of the human Brain. European Journal of Morphology. 2001;39(5):307-311(5).
Angelie Jessica S, Mukilan R. Study on microscopic vascular anatomy by luminal cast plastination of renal blood vessels using epoxy resin in bulls kidney. J. Pharm. Sci. and Res. 2019;11(6):2240-2242.
Abstract View: 74 times
PDF Download: 20 times