In vitro Antioxidant and Anticancer Activity of Cyanobacteria
Dina A. Abd El Sadek
Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City (USC), Sadat City 22857/79, Egypt
Ragaa A. Hamouda *
Department of Microbial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City (USC), Sadat City 22857/79, Egypt
Khalid Bassiouny
Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City (USC), Sadat City 22857/79, Egypt
Hala Elharoun
Department of Histology, Faculty of Medicine, Menoufia University, Egypt
*Author to whom correspondence should be addressed.
Abstract
Some cyanobacteria have therapeutic properties related to anticancer, antioxidant and antibacterial activity. The aim of the present study was to determine the polyphenol content together with antioxidant and anticancer activity against Ehrlich Ehrlich Ascites Carcinoma cell line in vitro of Anabaena oryzae, Calothrix marchica and Spirulina platensis, selecting the best alga for further investigations. The results indicate that methanol extracts of S. platensis has the greatest polyphenol content, together with the highest antioxidant and anticancer activity in vitro followed by A. oryzae and C. marchica respectively. Based on gas chromatography/mass spectrometry analysis of S. platensis methanol extract show that the most dominant content was octadecenoic acid methyl ester (ω- 9).
Keywords: Cyanobacteria, Spirulina platensis, antioxidant, polyphenol content, Ehrlich Ascites Carcinoma cell line (EAC)