Assessing the Impact of Onion Juice on the Levels of Leutinizing Hormone and Follicle Stimulating Hormone in Female Mice Exposed to Transfluthrin Smoke

Helen Anthony Waribo *

Department of Medical Laboratory Science.Rivers State University, Nkpolu Oroworukwo, P.M.B.- 5080, Port Harcourt, Rivers State, Nigeria.

MaryJane Oluomachi Emenike

Department of Medical Laboratory Science.Rivers State University, Nkpolu Oroworukwo, P.M.B.- 5080, Port Harcourt, Rivers State, Nigeria.

Ngozi Brisibe

Department of Medical Laboratory Science.Rivers State University, Nkpolu Oroworukwo, P.M.B.- 5080, Port Harcourt, Rivers State, Nigeria.

*Author to whom correspondence should be addressed.


Aim: Transfluthrin is a fast-acting insecticide used in household mainly against mosquites. Its uses have not been without health risk to humans. The study aims at evaluating the effect of onion juice on follicle stimulating hormone and leutinizing hormone levels in mice exposed to transfluthrin smoke.

Study Design: This is an animal study conducted in the Animal House, College of Medical Science in Rivers State University.

Materials and Methods: Thirty( 30) female mice were used in this study.  They were divided into six groups of five mice each; Group 1(negative control, exposed to normal environmental air), Group 2(exposed to 6g of transfluthrin coated insecticide paper smoke and given 0.2ml of onion juice), Group 3(exposed to 6g of transfluthrin coated insecticide paper smoke only), Group 4(exposed to 12g of transfluthrin coated insecticide paper smoke and given 0.2ml of onion juice), Group 5(exposed to 12g of transfluthrin coated insecticide paper smoke only), Group 6(given 0.2ml of onion juice only) through the oral gavage method daily. The smoke inhalation lasted for 15minutes  daily for 28 days using the already formulated packaged transflutrin coated insecticide papers in the market.    

Results: There was a significant difference in the mean values of Follicle Stimulating Hormone in groups 2, 3, 4, 5 & 6 when compared to group 1 (P=0.004).  There was no significant difference observed in the mean values of Leutinizing Hormone in the various groups (P=0.068).  The ovaries in groups 2, 3, 4, 5 & 6 showed several distortion and fluid congestion, which could be attributed to auto regulatory mechanisms leading to biochemical derangement.

 Conclusion: This study shows  that  the exposure significantly altered the hormonal levels and the ovarian cyto architecture of the mice. It also reveals that exposure to transfluthrin is detrimental to the reproductive functions of female mice however, the Onion juice administered to the mice in this study showed no ameliorative effect on the ovarian distortion, hence not improving the reproductive status of the mice.

Keywords: Transfluthrin smoke, LH, FSH, onion juice, fertility, reproductive health

How to Cite

Waribo , H. A., Emenike , M. O., & Brisibe, N. (2024). Assessing the Impact of Onion Juice on the Levels of Leutinizing Hormone and Follicle Stimulating Hormone in Female Mice Exposed to Transfluthrin Smoke. Asian Journal of Medicine and Health, 22(6), 168–177.


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Chrustek A, Hołyńska-Iwan I, Dziembowska I, Bogusiewicz J, Wróblewski M, Cwynar A, Olszewska-Słonina D. Current Research on the Safety of Pyrethroids Used as Insecticides. Medicina (Kaunas). 2018;54(4):61. DOI: 10.3390/medicina54040061. PMID: 30344292; PMCID: PMC6174339.

Masalu JP, Finda M, Killeen GF. Creating mosquito-free outdoor spaces using transfluthrin-treated chairs and ribbons. Malaria Journal. 2020;19:109. Available:

Masalu JP, Finda M, Okumu FO. Efficacy and user acceptability of transfluthrin-treated sisal and hessian decorations for protecting against mosquito bites in outdoor bars. Parasites Vectors. 2017;10: 197. Available:

4.Oyeniran DA, Ojewale AO, Jewo PI, Ashamu EA, Adeniyi OO, Adelakun SA. Infertility: A product of smoke emanating from Transfluthrin coated insecticide paper (TCIP). Toxicology Research and Application. 2021;5. DOI: 10.1177/23978473211025467

Bosch E, Alviggi C, Lispi M, Conforti A, Hanyaloglu AC, Chuderland D, Simoni M, Raine-Fenning N, Crépieux P, Kol S, Rochira V, D'Hooghe T, Humaidan P. Reduced FSH and LH action: implications for medically assisted reproduction. Human Reproduction. 2021;36(6): 1469-1480. DOI: 10.1093/humrep/deab065. PMID: 33792685; PMCID: PMC8129594.

Oduwole OO, Huhtaniemi IT, Misrahi M. The roles of luteinizing hormone, follicle-stimulating hormone and testosterone in spermatogenesis and folliculogenesis revisited. Int J Mol Sci. 2021;22(23): 12735. DOI: 10.3390/ijms222312735. PMID: 34884539; PMCID: PMC8658012

Raju GA, Chavan R, Deenadayal M, Gunasheela D, Gutgutia R, Haripriya G, Govindarajan M, Patel NH, Patki AS. Luteinizing hormone and follicle stimulating hormone synergy: A review of role in controlled ovarian hyper-stimulation. Journal of Human Reproductive Sciences. 2013;6(4):227-34. DOI: 10.4103/0974-1208.126285. PMID: 24672160; PMCID: PMC3963304.

Griffiths G, Trueman L, Crowther T, Thomas B, Smith B. Onions—A global benefit to health. Phytotherapy Research. 2002; 16(7):603–615.

Connors TA. Steering committee on identification of toxic and potentially toxic chemicals for consideration by the national toxicology program toxicity testing—strategies to determine needs and priorities. National Academy Press, Washington, 1984;382.

Ye X, Liu J. Effects of pyrethroid insecticides on hypothalamic-pituitary-gonadal axis: A reproductive health perspective. Environmental Pollution. 2019;245:590-599.

Saillenfait AM, Ndiaye D, Sabaté JP. Pyrethroids: Exposure and health effects–an update. International Journal of Hygiene and Environmental Health. 2015;218(3): 281-292.

Liu Y, Wang YL, Chen MH, Zhang Z, Xu BH, Liu R, Xu L, He SW, Li FP, Qi ZQ. Methoxychlor exposure induces oxidative stress and affects mouse oocyte meiotic maturation. Molecular Reproduction and Development. 2016;83:768–779.

Mandal J, Parija SC. Ethics of involving animals in research. Trop Parasitol. 2013; 3(1): 4-6. DOI: 10.4103/2229-5070.113884. PMID: 23961435. PMCID: PMC3745669.

Nicolopoulou-Stamati P, Pitsos MA. The impact of endocrine disrupters on the female reproductive system. Human Reproduction Update. 2001;7:323-330.

Khaki A, Fathiazad F, Nouri M, Khaki AA, Khamenehi HJ, Hamadeh M. Evaluation of androgenic activity of allium cepa on spermatogenesis in the rat. Folia Morphologica. 2009;68(1):45-51.

Zhao XX, Lin FJ, Li H, Li HB, Wu DT, Geng F, Ma W, Wang Y, Miao BH, Gan RY. Recent Advances in Bioactive Compounds, Health Functions, and Safety Concerns of Onion (Allium cepa L.). Frontiers in Nutrition. 2021;8:669805. DOI: 10.3389/fnut.2021.669805. PMID: 34368207; PMCID: PMC8339303.

Narges M, Vahideh G, Farzaneh S, Marzie B, Farzaneh K, Ramin R, Mohammad HB. A review of anti-inflammatory, antioxidant, and immunomodulatory effects of Allium cepa and its main constituents, Pharmaceutical Biology. 2021;59(1):285-300, DOI: 10.1080/13880209.2021.1874028

Neda M, Mehrdad M. The Effect of Red Onion Extract on Reproductive Hormones in Female Mice. The Pharmaceutical and Chemical Journal. 2020;7(2):132-137.

Marettova E, Maretta M, Legáth J. Effect of pyrethroids on the female genital system. Animal Reproduction Science. 2017;184: 132-138.

Jeje SO, Akpan EE, Kunle-Alabi OT, Akindele OO, Raji Y. Protective role of Allium cepa Linn (onion) juice on maternal dexamethasone-induced alterations in reproductive functions of female offspring of Wistar rats. Current Research in Physiology. 2021;4:145-154.

Khaki A, Khaki AA, Hajhosseini L, Golzar FS, Ainehchi N. The anti-oxidant effects of ginger and cinnamon on spermatogenesis dysfunction of diabetes rats. African Journal of Traditional, Complementary, and Alternative Medicines. 2017;14:214-223.

Patel S, Zhou C, Rattan S, Flaws JA. Effects of Endocrine-Disrupting Chemicals on the Ovary. Biol Reprod. 2015;93(1):20. DOI: 10.1095/biolreprod.115.130336. Epub 2015 Jun 10. PMID: 26063868; PMCID: PMC6366440.

Tian Qj, Zhao Xy, Wang Y. Histologic pattern is better correlated with clinical outcomes than biochemical classification in patients with drug-induced liver injury. Mod Pathol. 2019;32:1795–1805.


Gavrielides MA, Ronnett BM, Vang R, Sheikhzadeh F, Seidman JD. Selection of Representative Histologic Slides in Interobserver Reproducibility Studies: Insights from Expert Review for Ovarian Carcinoma Subtype Classification. J Pathol Inform. 2021;12:15.

DOI: 10.4103/jpi.jpi_56_20. PMID: 34012719; PMCID: PMC8112350.

Patrick L, George L, Natalie NC, Shih RE, Michael D, Anant M. Evaluating stability of histomorphometric features across scanner and staining variations: prostate cancer diagnosis from whole slide images. Journal of Medical Imaging. 2016;3(4): 047502. DOI: 10.1117/1.JMI.3.4.047502.

Ugljesa D, Gelareh, Z, Kenneth, A, Phedias D. Precision histology: How deep learning is poised to revitalize histomorphology for personalized cancer care. Lancet. 2017;3(2):1691-1698.