Evaluation of the Effects of Pediococcus acidilactici Isolated from Wara, a Nigerian Milk Product, in the Prevention of Diarrhea and the Modulation of Intestinal Microflora in Wistar Rats

Main Article Content

Temidayo Emmanuel Olajugbagbe
Samson Oludare Ayodeji Odukoya
Bridget Okiemute Omafuvbe

Abstract

Animals have been used in the evaluation of probiotic potentials of lactic acid bacteria for the development of functional food as only in-vitro tests may not be enough to ascertain the probiotic ability of an organism and its safety in a living host. The action of a probiotic Pediococcus acidilactici strain isolated from Wara, a Nigerian milk product, in the treatment of Diarrhea in wistar rats infected orally with Diarrhoeagenic Enterotoxigenic Escherichia coli as well as the survival and microflora modulation of the probiotic strain in the gastrointestinal tract were evaluated. Five groups of seven rats each were infected and treated as the case may be with the pathogen and probiotic respectively. Each group received specific treatments for 30 days, during which the animals were closely monitored and the faecal samples were analyzed for the trend of E. coli and LAB counts. Also, specific organs of the Gastrointestinal tract (GIT) were examined for any histomorphological disparity after 30 days of daily treatment. Clinical signs were observed in the pathogen challenged animals while the probiotic treated groups displayed less pronounced Diarrheal symptoms and a negligible E. coli count. The strain adhered to the mucosal wall and did not initiate any adverse effect on the organs of the GIT after treatment. It was concluded that Pediococcus acidilactici isolated from Wara, a Nigerian milk product, was able to improve gut health through pathogen exclusion and had no adverse effect on the general health of the host.

Keywords:
Probiotic, Pediococcus acidilactici, Wara, diarrhea, Escherichia coli

Article Details

How to Cite
Olajugbagbe, T. E., Odukoya, S. O. A., & Omafuvbe, B. O. (2020). Evaluation of the Effects of Pediococcus acidilactici Isolated from Wara, a Nigerian Milk Product, in the Prevention of Diarrhea and the Modulation of Intestinal Microflora in Wistar Rats. Asian Journal of Medicine and Health, 18(9), 94-106. https://doi.org/10.9734/ajmah/2020/v18i930240
Section
Original Research Article

References

Joint Food and Agriculture Organization and World Health Organization (FAO/WHO). Working Group Report on Drafting Guidelines for the Evaluation of Probiotics in Food. London, Ontario, Canada. 2002;1-11.

Tezel BU. Preliminary in-vitro evaluation of the probiotic potential of the bacteriocinogenic strain Enterococcus lactis PMD74 isolated from ezine cheese. J. Food Qual. 2019;1-12. Available:https://doi.org/10.1155/2019/4693513

Cho JH, Zhao PY, Kim IH. Probiotics as a dietary additive for pigs. J. Anim. Vet. Adv. 2011;10(16):2127-2134. DOI: 10.3923/javaa.2011.2127.2134

Yirga H. The use of probiotics in animal nutrition. J Prob Health. 2015;3:132. DOI: 10.4172/2329-8901.1000132

Varankovich NV, Nickerson MT, Korber DR. Probiotic based strategies for therapeutic and prophylactic use against multiple gastrointestinal diseases. Frontier Microbiol. 2015;6:685. DOI: 10.3389/fmicb.2015.00685

Peter AK, Umar U. Combating diarrhea in Nigeria: The way forward. J. Microbiol Exp. 2018;6(4):191-197. DOI: 10.15406/jmen.2018.06.00213

Roussel C, Cordonnier C, Livrelli V, Van de Wiele T. Blanquet-Diot S. Enterotoxigenic and enterohemorrhagic Escherichia coli: survival and modulation of virulence in the human gastrointestinal tract. Escherichia coli-Recent Advances on Physiology, Pathogenesis and Biotechnology Applications. 2017;1:3-24. Available:http://dx.doi.org/10.5772/intechopen.68309

Ferreira AF, Pereira-Manfro WF, Rosa ACP. Diarrhoeagenic Escherichia coli and probiotic activity against food-borne pathogens: A brief review. Gastroenterol. Hepatol. 2017;7(5):00248. DOI: 10.15406/ghoa.2017.07.00248

Mohammadi M, Tajabadi EM, Pakpour B. Restoration of morphine-induced amnesia by Pediococcus acidilactici, an indigenous probiotics of Iran in male wistar rats. Arch. Med. Lab. Sci. 2018;4(1):1-8.

Wang S, Yao B, Gao H, Zang J, Tao S, Zhang S, et al. Combined supplementation of Lactobacillus fermentum and Pediococcus acidilactici promoted growth performance, alleviated inflammation and modulated intestinal microbiota in weaned pigs. BMC Vet. Res. 2019;15(239):1-11. Available:https://doi.org/10.1186/s12917-019-1991-9

Abbasiliasi S, Tan JS, Bashokouh F. In vitro assessment of Pediococcus acidilactici kp10 for its potential use in the food industry. BMC Microbiol. 2017; 17(121):1-11. DOI: 10.1186/s12866-017-1000-z

Gupta A, Sharma N. Characterization of potential probiotic lactic acid bacteria- Pediococcus acidilactici Ch-2 Isolated from Chuli- A traditional apricot product of Himalayan region for the production of novel bioactive compounds with special therapeutic properties. J Food Microbiol Saf Hyg. 2017;2:119. DOI: 10.4172/2476-2059.1000119

Brousseau JP, Talbot G, Beaudoin F, Lauzon K, Roy D, Lessard M. Effects of probiotic Pediococcus acidilactici strain MA18/5M and Saccharomyces cerevisae subsp. boulardii strain SB-CNCM 1-1079 on feacal and intestinal microbiota of nursing and weaning piglets. Journal of Animal Science. 2015;93(11):5313-26. DOI: 10.2527/jas.2015-9190

Shukla G, Sharma G, Goyal N. Probiotic characterization of lactobacilli and yeast strains isolated from whey beverage and therapeutic potential of lactobacillus yoghurt in Murine Giardiasis. Am. J. Biomed. Sci. 2010;2(3):248-261. DOI: 10.5099/aj100300248

Avwioro OG. Tissue processing. Histochemistry and Tissue Pathology. 1st Edition. 2002;218-219 ISBN 978 35627-9-7.

Oghenesuvwe EE, Tedwins JO, Obiora IS, Lotanna AD, Ujam N, Okezie M. et al. Preclinical screening techniques for anti-diarrhoeal drugs: A comprehensive review. Am. J. Physiol. Biochem. Pharmacol. 2018;7(2):61–74. DOI: 10.5455/ajpbp.20180329014330

Raj V, Rai A, Singh M. Detection of bioflavonoids from methanol bark extracts of acacia catechu willd through HPTLC and their role as antidiarrhoeal agents. J. Analytical Pharmacol. Res. 2017;5(4): 00146. DOI: 10.15406/japlr.2017.05.00146

Rizzatti G, Lopetuso LR, Gibiino G, Binda C. Gasbarrini A. Proteobacteria: A common factor in human diseases. Biomed Res. Int. 2017;1-7. Available:doi.org/10.1155/2017/9351507

Holy O, Chmelar D. Oxygen tolerance in anaerobic pathogenic bacteria. Folia Microbiol (Praha). 2012;57(5):443-446. DOI: 10.1007/s12223-012-0149-y

Gasmalla M, Khadir K, Musa A, Aboshora W, Zhao W. Evaluation of some physicochemical parameters of three commercial milk products. Pak. J. Food Sci. 2013;23(2):62-65. ISSN: 2226-5899.

Samanta A, Mandal A, Patra A, Mandal S, Roy S, Pradhan S. et al. Impact of probiotic supplementation on intestinal microflora of rat under environmental hypobaric pressure. Int. J. Curr. Microbiol. Appl. Sci. 2014;3(8):282-291.

Shyamala G, Meenambigai P, Prabhavathi P, Raja R, Yesudoss L. Probiotics and its effects on human health- A review. International J. Curr. Microbiol. Appl. Sci. 2016;5(4):384-392. Available:http://dx.doi.org/10.20546/ijcmas.2016.504.046

Villena J, Kitazawa H. Probiotic microorganisms; A closer look. Microorganisms. 2017;5(17):1-3. DOI: 10.3390/microorganisms5020017

Gombosova L, Lazurova I, Zakuciova M, Curova K, Kmetova M, Petrasova D. Siegfried L. genes of intestinal Escherichia coli and their relation to the inflammatory activity in patients with ulcerative colitis and Chron’s disease. Folia Microbiol. 2011;56:367-372. DOI: 10.1007/s12223-011-0051-z

Gibiino G, Lopetuso LR, Scaldeferri F, Rizzatti G, Binda C, Gasbarrini A. Exploring bacteriodetes: Metabolic key points and immunological tricks of our gut commensals. Dig. Liver. Dis. 2018;50(7): 635-639. Available:https://doi.org/10.1016/j.dld.2018.03.016

Fischer AH, Jacobson KA, Rose J, Zeller R. Hematoxylin and eosin staining of tissue and cell sections. CSH Protoc; 2008. DOI: 10.1101/pdb.prot4986

Gomes AT, Elias P, Scaletsky CA, Guth EC, Rodrigues F, Piazza MF, Ferreira CS, Martinez B. Diarrhoeagenic Escherichia coli. Br J. Microbiol. 2016;47(1):3–30. Available:http://dx.doi.org/10.1016/j.bjm.2016.10.015

Afsahi A, Mahmoudi H, Ebrahimi A, Aeini Z, Esmaeili D. Evaluation of the effect of Lactobacillus plantarium probiotics produced from broad bean seed in prevention of Helicobacter pylori in stomach tissue of C57BL/6 mice. J. Can. Sci. Ther. 2018;10(4):085-092. DOI: 10.4172/1948-5956.1000524